BI337L Biochemistry Laboratory

for FA 2010

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BI 337L Biochemistry Lab


FA 2010 HO


Royals, Brenda R.


Instructor of Biology


M.S. in Biochemistry and Molecular Biology, LSU Health Sciences Center

Office Location

113A, Science Hall

Office Hours

M 9-10:00; T 8-10:00 & 2-3:00; W 9-11:00; R 9-10:00; F 8-9:00

Daytime Phone

(816) 584-6592


Semester Dates

August 16, 2010 - December 10, 2010

Class Days


Class Time



CH318 and CH318L and/or the equivalent

Credit Hours



Biochemistry: A Short Course by Tymoczko, Berg, & Stryer, 1st edition, 2010, WH Freeman & Co. New York, NY (ISBN-13: 978-0-7167-5840-2).

Supplemental handouts for each laboratory exercise will also be provided by the instructor via eCompanion or during class.

Textbooks can be purchased through the MBS bookstore

Textbooks can be purchased through the Parkville Bookstore

Additional Resources:

More advanced textbooks always contain more detailed information, and some of these are available in the library. Additional information, informational links, suggested readings, and supplemental lab exercises will be presented in class or online as they become available. All official class announcements and outlines will be posted on the E-Companion site (  It is therefore crucial that each student learn how to navigate this important site.  Postings may be in the form of power point, word documents, or excel files.  Each student is responsible for printing notes and outlines that may be provided by the instructor.

McAfee Memorial Library - Online information, links, electronic databases and the Online catalog. Contact the library for further assistance via email or at 800-270-4347.
Career Counseling - The Career Development Center (CDC) provides services for all stages of career development.  The mission of the CDC is to provide the career planning tools to ensure a lifetime of career success.
Park Helpdesk - If you have forgotten your OPEN ID or Password, or need assistance with your PirateMail account, please email or call 800-927-3024
Resources for Current Students - A great place to look for all kinds of information

Course Description:

This is a co-requisite laboratory course to accompany the biochemistry lecture. This course is designed for both biology and chemistry majors.  

Educational Philosophy:

Learning is an active and interactive process.  Ideally, it would best be promoted by a cobitnation of questioning and feedback involving each student.  Thus, I like to teach in ways to try and reach all types of students with their diverse learning styles and do my best to provide a constructive learning environment.  I like to enourage students to set high learning expectations, thus classroom attendance and participation are necessary for achievement of this goal.   

Materials learned in the lecture portion of the course will be applied in the laboratory exercises. It is therefore crucial that students attend and participate in each laboratory session.  Hands-on laboratory work will expand and reinforce many of the major concepts from lecture so that you remember them in your future career settings.
I have an open door policy and I encourage students to come by my office any time they have questions or feel they need additional help. I am here to help you in any way that I can.

Class Assessment:

100 points
Mid-Term Exam                  
125 points
Final Exam   
175 points
Lab Reports (50 pts ea.)
600 points
Total Points   
 1000 points
There will be two laboratory examinations over the laboratory exercises performed during the designated class period.  Exams will contain multiple-choice, short answer, matching, and a few discussion question.  Each of these exams will cover the lab exercises up to the exam date.  The final exam is not comprehensive.  A detailed lab report for each laboratory excerise will be worth 50 points per report and will be due one week after the excercise is completed.  A summary of the total possible points is shown above.


A traditional grading scale will be followed with: 90% or greater being an A, 80% or greater being a B, 70% or greater for a C, 60% or greater for a D, and less than 60% being an F.  Your cumulative percentage in the class is simply your accumulated points divided by the total points possible and then multiplied by 100%. 

Late Submission of Course Materials:

Late homework and assignments (including lab notebooks when called in for review or lab projects with a dated deadline) will be reduced in credit by 25% for each day that the work is late. Students missing class due to participation in athletic or other university-sponsored events must turn in all due assignments PRIOR to their expected absence (see below).

Classroom Rules of Conduct:

All students are expected to follow the common rules of acceptable and courteous behavior while in class, illustrating respect for each other and the instructor.  Any disruptive behavior will not be allowed.
Cell phones and pagers must be turned off or put on silent mode prior to entering the classroom.  Text messaging during class will result in immediate dismissal.


Course Topic/Dates/Assignments:

                Tentative Schedule
                     (Subject to changes made by the instructor.)         


Laboratory Exercise

Aug. 18

Check In/ Lab Policies/ Biochem. Basics

Aug. 25

Carbohydrates and Lipids

Sept. 1

Anthocyanins/ Hydrolysis of Red Roses & Cabbage

Sept. 8

Analysis of Amino Acids

Sept. 15

Protein Determination

Sept. 22

Size Exclusion Chromatography

Sept. 29

Introduction to Electrophoresis 

Oct. 6

Mid-Term Exam

Oct. 13


Oct. 20

Proteomics-Western Blot I

Oct. 27

Proteomics-Western Blot II

Nov. 3

DNA Isolation

Nov. 10

Restriction Enzyme Analysis of DNA

Nov. 17


Nov. 24


Dec. 1

Final Exam


Academic Honesty:
Academic integrity is the foundation of the academic community. Because each student has the primary responsibility for being academically honest, students are advised to read and understand all sections of this policy relating to standards of conduct and academic life. Park University students and faculty members are encouraged to take advantage of the University resources available for learning about academic honesty ( or Park University 2010-2011 Undergraduate Catalog Page 92
       Please do not share or copy answers for any assignment, including lab write-ups, exam answers, and lab projects. Students should never copy verbatim from books, manuals, or internet sources (see below). Do not  copy from someone else's exam paper or whisper answers to each other during exams. (Both students will receive a 0 for that exam.)

Plagiarism involves the use of quotations without quotation marks, the use of quotations without indication of the source, the use of another's idea without acknowledging the source, the submission of a paper, laboratory report, project, or class assignment (any portion of such) prepared by another person, or incorrect paraphrasing. from Park University 2010-2011 Undergraduate Catalog Page 92-93
PLEASE be careful not to plagiarize; we have several programs that will easily detect such behavior within the patterns of your written work.

Attendance Policy:
Instructors are required to maintain attendance records and to report absences via the online attendance reporting system.

  1. The instructor may excuse absences for valid reasons, but missed work must be made up within the semester/term of enrollment.
  2. Work missed through unexcused absences must also be made up within the semester/term of enrollment, but unexcused absences may carry further penalties.
  3. In the event of two consecutive weeks of unexcused absences in a semester/term of enrollment, the student will be administratively withdrawn, resulting in a grade of "F".
  4. A "Contract for Incomplete" will not be issued to a student who has unexcused or excessive absences recorded for a course.
  5. Students receiving Military Tuition Assistance or Veterans Administration educational benefits must not exceed three unexcused absences in the semester/term of enrollment. Excessive absences will be reported to the appropriate agency and may result in a monetary penalty to the student.
  6. Report of a "F" grade (attendance or academic) resulting from excessive absence for those students who are receiving financial assistance from agencies not mentioned in item 5 above will be reported to the appropriate agency.

Park University 2010-2011 Undergraduate Catalog Page 95-96
It is MANDATORY that you attend all class meetings and all scheduled laboratories. Part of a university education is preparation for professional conduct in your future life. Attendance at work or graduate/professional school is mandatory -- if you wish to remain employed or enrolled.
     In addition, education is a cooperative effort, and you are as responsible for furthering the education of your fellow classmates and the instructor as the instructor is for educating you. Get in the habit of being here on time, for this is a behavior you will need to cultivate if you wish to succeed.

   Be advised that a student is always responsible for any class material, whether they are present during a particular class session -- or not. It is the responsibility of the student to obtain notes and any notice of assignments due or announcements made on their own.

    There are NO EXCUSED ABSENCES in this course for any reason (medical, personal, job-related, mechanical failure, death in the family, death of a beloved pet, death of ambition, and so forth). However, since personal emergencies may nonetheless occur during the semester, your first four (4) absences (lecture and/or lab) are free and will draw you no penalty.
    HOWEVER, with each additional absence past the first four, 5% will be deducted from the student's final overall grade for the course. Furthermore, eight (8) absences in a twice-a-week semester class is an excessive number, and you will automatically be given an F after you miss eight (8) lecture or lab sessions (for ANY reason).

   TARDINESS or LEAVING:  Quizzes are typically given within the first 10 minutes or the last 10 minutes of class. If you miss them, these can never be made up. Some quizzes are “bonus” – meaning that you can actually gain extra points by knowing something; other quizzes are evaluative – meaning that you will lose points if you don't know all the answers.

     EXCEPTION FOR SCHOOL ACTIVITIES:  Athletes who miss class for an athletic event or contest or students who miss class due to  other school sponsored events will not be penalized IF AND ONLY IF the instructor is GIVEN WRITTEN NOTICE ONE WEEK IN ADVANCE -- and -- BOTH WORK ASSIGNMENTS AND EXAMS ARE TAKEN BEFORE ACTUAL ABSENCE!

Disability Guidelines:
Park University is committed to meeting the needs of all students that meet the criteria for special assistance. These guidelines are designed to supply directions to students concerning the information necessary to accomplish this goal. It is Park University's policy to comply fully with federal and state law, including Section 504 of the Rehabilitation Act of 1973 and the Americans with Disabilities Act of 1990, regarding students with disabilities. In the case of any inconsistency between these guidelines and federal and/or state law, the provisions of the law will apply. Additional information concerning Park University's policies and procedures related to disability can be found on the Park University web page: .
Although you have the right to keep all conditions private, my desire is to help anyone who has any sort of trouble taking the class. If you come and talk with me about known problems, we can make certain that you are getting the help you deserve.


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Last Updated:8/5/2010 12:54:45 PM